新型冠状病毒假病毒的构建及其在血清中和抗体测定中的应用
梁婉欣1, 刘淑燕2, 段炼2, 周雪峰3, 邬林枫3, 刘文齐2, 张更伟2, 朱华晨1,4,*, 张国良2,*

Construction of SARS-CoV-2 pseudovirus and its application in measuring the neutralization activity of serum antibodies
LIANG Wan-xin1, LIU Shu-yan2, DUAN Lian2, ZHOU Xue-feng3, WU Lin-feng3, LIU Wen-qi2, ZHANG Geng-wei2, ZHU Hua-chen1,4, ZHANG Guo-liang2
SARS-CoV-2假病毒表达S蛋白的Western blot鉴定 A.细胞上清,B.细胞裂解液。1.共转染质粒组（pNL4-3.Luc.R-E- 4 μg + pcDNA3.1-S 1 μg）;2.骨架质粒组（pNL4-3.Luc.R-E- 4 μg）; 3.表达质粒组（pcDNA3.1-S 1 μg）;4 .空白对照组（不转染质粒）。